کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4160011 1273836 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The evidence of maternal microchimerism in biliary atresia using fluorescent in situ hybridization
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پریناتولوژی (پزشکی مادر و جنین)، طب اطفال و بهداشت کودک
پیش نمایش صفحه اول مقاله
The evidence of maternal microchimerism in biliary atresia using fluorescent in situ hybridization
چکیده انگلیسی

BackgroundBiliary atresia (BA) is a cholestatic disease of unknown etiology. It has recently been suggested that graft-vs-host disease caused by microchimerism is an etiology in the development of autoimmune disease. Moreover, the liver is a frequent target organ of graft-vs-host disease. The aim of this study is to identify the presence and extent of maternal microchimerism and to determine whether it plays a role in the etiology of BA.MethodsThe liver biopsy specimens of 6 male patients with BA (BA group) and 6 males with other liver diseases (non-BA group) were assayed for X- and Y-chromosome using fluorescent in situ hybridization. The cells with 2 sex chromosomes in the nuclei were counted. Cells with 1 X- and 1 Y-chromosomes were considered to be host cells, and those with 2 X-chromosome were considered to be of maternal origin.ResultsThe frequency of cells with XX chromosomes per 1000 host cells in the BA group and the non-BA group were 3.00 ± 0.75 and 0.99 ± 0.50, respectively (P = .005). Moreover, the age at the time of biopsy did not affect the number of chimeric cells.ConclusionThe presence of female cells in the liver of male patients with BA was significantly higher than in males with other liver disease. Maternal microchimerism is therefore suggested to contribute to the pathogenesis of BA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pediatric Surgery - Volume 42, Issue 12, December 2007, Pages 2097–2101
نویسندگان
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