کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4201929 1279432 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enhanced Type III Secretion System Expression of Atypical Shigella flexneri II:(3)4,7(8)
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی سیاست های بهداشت و سلامت عمومی
پیش نمایش صفحه اول مقاله
Enhanced Type III Secretion System Expression of Atypical Shigella flexneri II:(3)4,7(8)
چکیده انگلیسی

ObjectivesWe aimed at evaluating the virulence of atypical Shigella flexneri II:(3)4,7(8) by DNA microarray and invasion assay.MethodsWe used a customized S. flexneri DNA microarray to analyze an atypical S. flexneri II:(3)4,7(8) gene expression profile and compared it with that of the S. flexneri 2b strain.ResultsApproximately one-quarter of the atypical S. flexneri II:(3)4,7(8) strain genes showed significantly altered expression profiles; 344 genes were more than two-fold upregulated, and 442 genes were more than 0.5-fold downregulated. The upregulated genes were divided into the category of 21 clusters of orthologous groups (COGs), and the “not in COGs” category included 170 genes. This category had virulence plasmid genes, including the ipa-mxi-spa genes required for invasion of colorectal epithelium (type III secretion system). Quantitative reverse-transcription polymerase chain reaction results also showed the same pattern in two more atypical S. flexneri II:(3)4,7(8) strains. Atypical S. flexneri II:(3)4,7(8) showed four times increased invasion activity in Caco-2 cells than that of typical strains.ConclusionOur results provide the intracellularly regulated genes that may be important for adaptation and growth strategies of this atypical S. flexneri.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Osong Public Health and Research Perspectives - Volume 3, Issue 4, December 2012, Pages 222–228
نویسندگان
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