کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4209368 1280482 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct antimicrobial susceptibility testing method for analysis of sputum collected from patients with cystic fibrosis
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی ریوی و تنفسی
پیش نمایش صفحه اول مقاله
Direct antimicrobial susceptibility testing method for analysis of sputum collected from patients with cystic fibrosis
چکیده انگلیسی

BackgroundChronic Pseudomonas aeruginosa colonisation and subsequent exacerbations in patients with cystic fibrosis (CF) require antimicrobial treatment. But since multiple morphotypes and other Gram-negative bacteria with different antibiotic susceptibilities are often isolated inside the same sputum sample, bacteriological analysis is difficult.MethodsTo simplify this analysis, we explored a direct sputum antimicrobial susceptibility testing (DSST) method by applying E test directly on plates inoculated with the sputum. A total of 316 samples collected from CF patients were analysed and compared with standard procedures (SP) for the identification and antimicrobial susceptibility testing of all Gram-negative bacterial species.ResultsDSST was as efficient as SP to detect P. aeruginosa including the mucoid morphotype in monomicrobial specimen, but was less sensible to detect all Gram-negative bacteria present in the same sample. It allowed the direct reading of the MIC inhibiting all Gram-negative bacteria. Agreements between these global MICs with the cumulative antibiotics susceptibility of all Gram-negative bacteria measured by SP were excellent for tobramycin and imipenem (> 96%) and satisfactory for ticarcillin, ceftazidime, aztreonam and ciprofloxacin (90.4% to 94.3%).In conclusion, the DSST method is an efficient and easy antibiotic susceptibility testing method.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Cystic Fibrosis - Volume 7, Issue 3, May 2008, Pages 238–243
نویسندگان
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