کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4209795 1280498 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dysregulated interleukin-8 secretion and NF-κB activity in human cystic fibrosis nasal epithelial cells
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی ریوی و تنفسی
پیش نمایش صفحه اول مقاله
Dysregulated interleukin-8 secretion and NF-κB activity in human cystic fibrosis nasal epithelial cells
چکیده انگلیسی

BackgroundIt is not clear whether cystic fibrosis (CF) airway inflammation is a consequence of bacterial infection or is intrinsically dysregulated. The aim of this study was to investigate IL-8 secretion and NF-κB activity in primary respiratory epithelial cells cultured from nasal polyps obtained from CF and non-CF subjects.MethodsNF-κB activity was studied by electrophoretic mobility-shift and quantitative colorimetric assays in nuclear extracts. Immunoreactive IL-8 levels were assessed by ELISA in cell culture supernatants. Both parameters were studied at baseline and following challenge with Pseudomonas aeruginosa or stimulation with pro-inflammatory cytokines.ResultsUnder basal conditions, CF cells presented a significant higher activity of NF-κB than non-CF cells (P = 0.0004). P. aeruginosa challenge and IL-1β/H2O2 co-stimulation caused four and two fold induction of NF-κB activity in non-CF and CF cells, respectively. IL-8 levels in unstimulated CF cells were significantly higher than in non-CF cells (P = 0.0025). Upon incubation with P. aeruginosa and IL-1β/H2O2, non-CF cells produced 6.3 times more IL-8 than unstimulated cells, whereas IL-8 secretion increased only of 1.4 times in CF cells.ConclusionsCF respiratory epithelial cells exhibit a basal dysregulated production of IL-8 that partially correlates to enhanced NF-κB activity. Our data corroborate the hypothesis of a basal exaggerated inflammatory response in the CF respiratory epithelium.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Cystic Fibrosis - Volume 5, Issue 2, May 2006, Pages 113–119
نویسندگان
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