کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4256149 | 1284511 | 2013 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Induction of CD4+CD25+ T Cells and Control of Cardiac Allograft Rejection by CD40/CD40L Costimulatory Pathway Blockade in Mice Induction of CD4+CD25+ T Cells and Control of Cardiac Allograft Rejection by CD40/CD40L Costimulatory Pathway Blockade in Mice](/preview/png/4256149.png)
BackgroundRejection is a major problem in organ transplantation. We speculated that dendritic cells (DCs) with low expression of CD40 may inhibit T-cell responses providing protective effects on organ transplantions.MethodsRNA interference (RNAi) is an effective approach to degrade RNA levels of a target gene. We used RNAi to reduce CD40 levels in mouse bone marrow-derived DCs. Allogeneic T-lymphocyte proliferation stimulated by DCs was assessed in mixed lymphocyte reactions. A mouse model of heterotopic abdominal heart transplantation was performed to evaluate survival times and histologic grade of acute rejection at 7 days after transplantation. CD4+CD25+ regulatory T cells (Tregs) in peripheral blood were quantified by flow cytometry.ResultsMouse DCs with reduced CD40 expression lowered the proliferation of recipient T cells. More DCs with silenced CD40 delayed cardiac allograft rejection.ConclusionsReduction of CD40 in DCs induced peripheral Tregs and delayed cardiac allograft rejection.
Journal: Transplantation Proceedings - Volume 45, Issue 2, March 2013, Pages 611–617