کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4257548 | 1284544 | 2012 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A Model of Isolated, Vascular Whole Thymus Transplantation in Nude Rats A Model of Isolated, Vascular Whole Thymus Transplantation in Nude Rats](/preview/png/4257548.png)
BackgroundWe used a model of vascularized thymus lobes as a whole isolated organ transplantation in rats.Materials and MethodsMale Fisher rats (F344, RT11v1; n = 10) and male homozygous Rowett nude rats (rnu/rnu; n = 10) were used as donors and recipients, respectively. Both vascular lobes of the thymus as a whole isolated organ were heterotopically transplanted to the neck of recipients. The right common carotid artery of the donor thymus was anastomosed end-to-end to the homonymous artery of the recipient. The anterior vena cava and the left brachiocephalic vein of the donor thymus were anastomosed end-to-side to the right and left external jugular veins of the recipient, respectively. Histological examination was used to monitor graft viability; graft function was assessed using flow cytometry (FCM) and immunologic effects by skin grafts in vivo.ResultsAll recipients survived. Preparation of the donors and recipients took 35.6 ± 5.5 minutes and 60.3 ± 15.1 minutes, respectively. The blood supply to the thymus graft was patent. Histology of the thymus on postoperative days 14, 56, and 112 revealed viable grafts with preserved microarchitecture. FCM analysis showed 37.18 ± 11.1% CD3+ T cells at day 21 after transplantation. Skin grafts from F344 and Rowett rats survived 8–10 and more than 30 days, respectively, whereas all third-party Sprague Dawley grafts were rejected within 5 days.ConclusionWe developed a novel model of isolated, direct vascularized whole thymus transplantation in nude rats, in which both lobes of the fully vascularized thymus were harvested en bloc for successful transplantation.
Journal: Transplantation Proceedings - Volume 44, Issue 5, June 2012, Pages 1394–1398