کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4259159 | 1284567 | 2013 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The Early Protective Effect of Glutamine Pretreatment and Ischemia Preconditioning in Renal Ischemia–Reperfusion Injury of Rat The Early Protective Effect of Glutamine Pretreatment and Ischemia Preconditioning in Renal Ischemia–Reperfusion Injury of Rat](/preview/png/4259159.png)
BackgroundHeat shock proteins (HSP) play an important role in protecting cells against stress.MethodsUsing a rat model, we tested the hypothesis that pretreatment with glutamine (Gln) and ischemia preconditioning (IPC) increase the expression of HSP resulting in attenuation of renal ischemia/reperfusion (I/R) injury. Sprague-Dawley rats were randomized into 4 groups [group I, Gln injection (+), IPC (+); group II, Gln injection (+), IPC (−); group III, saline injection (+), IPC (+); group IV, saline injection (+), IPC (−)]. Renal HSP70 expression was determined by Western blotting and kidney function was assessed by blood urea nitrogen and serum creatinine. Renal cross-sections were microscopically examined for tubular necrosis, exfoliation of tubular epithelial cells, cast formation, and monocyte infiltration.ResultsGln pretreatment increased intrarenal HSP expression (P = .031). In group I, tubulointerstitial abnormalities were clearly slighter compared with the other groups (P < .001).ConclusionOur experiments suggest that (1) a single dose of Gln could induce HSP expression and (2) IPC could relieve renal I/R injury. In addition, IPC combined with Gln pretreatment had a synergic protective effect against renal I/R injury.
Journal: Transplantation Proceedings - Volume 45, Issue 9, November 2013, Pages 3203–3208