کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4260702 | 1610670 | 2008 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Donor Bone-Marrow Suppressor of Cytokine Signaling-1-Silenced Dendritic Cells Prolong Rat Intestinal Allograft Survival
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موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
عمل جراحی
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![عکس صفحه اول مقاله: Donor Bone-Marrow Suppressor of Cytokine Signaling-1-Silenced Dendritic Cells Prolong Rat Intestinal Allograft Survival Donor Bone-Marrow Suppressor of Cytokine Signaling-1-Silenced Dendritic Cells Prolong Rat Intestinal Allograft Survival](/preview/png/4260702.png)
چکیده انگلیسی
Suppressor of cytokine signaling (SOCS) molecules belong to intracellular proteins that inhibit Janus kinase as well as signal transduction and activators of transcription pathways. In this study, we investigated whether SOCS-1-silenced dendritic cells (DCs) prolonged allograft survival in rat intestinal transplantation. Donor bone marrow-derived DCs were genetically transfected with SOCS-1 siRNA using liposomes. The level of SOCS-1 expression was quantitated by Western blots. DC function was assessed by MTT in mixed leukocyte reactions. We injected donor-derived SOCS-1 silenced DCs 7 days before heterotopic intestinal transplantation between SD donors and Wistar recipients. We compared untransfected DCs and silenced DCs to suppress allogeneic mixed leukocyte reactions. Recipients pretreated with SOCS-1-silenced DCs showed moderate survival prolongation with a mean allograft survival of 18.3 ± 5.3 days (P < .05), compared with 6.4 ± 2.0 days in the control group and 7.2 ± 2.1 days in a control siRNA transfection DC group. The difference between untreated DCs group and the control group was not significant. In summary, SOCS-1 silenced DCs induced allogeneic T-cell hyporesponsiveness in vitro, promoting allograft survival in rat intestinal transplantation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Transplantation Proceedings - Volume 40, Issue 10, December 2008, Pages 3707-3710
Journal: Transplantation Proceedings - Volume 40, Issue 10, December 2008, Pages 3707-3710
نویسندگان
C. Zhu, H. Xu, J. Zhang, K. Wang, P. Zhu,