کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4261135 | 1284591 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Autonomic Dysfunction of Rat Jejunum Submitted to Cold Ischemic Preservation Is Prevented by Heparin
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موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
عمل جراحی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Autonomic Dysfunction of Rat Jejunum Submitted to Cold Ischemic Preservation Is Prevented by Heparin Autonomic Dysfunction of Rat Jejunum Submitted to Cold Ischemic Preservation Is Prevented by Heparin](/preview/png/4261135.png)
چکیده انگلیسی
Previous studies suggest that cold ischemic preservation (CIP) employed in small bowel transplantation promotes loss of intestinal motility due to severe lesions in autonomic enteric nerves. This autonomic dysfunction may be prevented by antioxidant agents. In this work, we investigated whether preservation with heparin prevented autonomic dysfunction of rat jejunum submitted to CIP for a long time. Jejunal segments (2 cm) of Wistar rats (12 to 16 weeks old) were preserved at 4°C in Ringer's lactate solution without (â) or with (+) 100 UI/mL heparin (H). After preservation for 12 hours, H+ and Hâ preparations were mounted in parallel in isolated organ baths containing 10 mL Tyrode's solution at 37°C for the study of neurogenic contractions evoked by electrical field stimulation (EFS; 10-30 Hz, 1-ms duration, 60 V) or by stimulation of nicotinic (NIC) or muscarinic (carbachol, CCh) cholinoceptors. The effects of NIC (hexamethonium, HEX) and muscarinic (atropine, ATR) antagonists were studied on these contractions. Contractions induced by EFS (30 Hz) were four times greater in H+ (1.02 ± 0.12 g) versus Hâ (0.26 ± 0.07 g), while contractions induced by NIC (1 mmol/L) were also four times higher in H+ (1.07 ± 0.10 g) than Hâ (0.25 ± 0.09 g) preparations. In addition, contractions induced by CCh (1 mmol/L) were two times higher in H+ (1.21 ± 0.13 g) than in Hâ (0.65 ± 0.10 g). EFS, NIC, and CCh contractions were inhibited by pretreatment of jejunum with HEX or ATR (1 μmol/L/30 min), in H+ and Hâ. These results indicated that addition of heparin to a preservation solution attenuated the autonomic dysfunction of rat jejunum submitted to CIP for a long time.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Transplantation Proceedings - Volume 38, Issue 6, JulyâAugust 2006, Pages 1779-1783
Journal: Transplantation Proceedings - Volume 38, Issue 6, JulyâAugust 2006, Pages 1779-1783
نویسندگان
M.O. Taha, M.M. Fraga, F.A. Guimarães, A. Jurkiewicz, A. Caricati-Neto,