کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4261264 1284592 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Better Induction and Differentiation Strategy for Rat Pancreatic Stem Cells: Transplant in Liver Niche
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
پیش نمایش صفحه اول مقاله
Better Induction and Differentiation Strategy for Rat Pancreatic Stem Cells: Transplant in Liver Niche
چکیده انگلیسی

Current in vitro induction protocols cannot generate mature islet cells from stem cells. Transplantation into the liver niche may greatly contribute to the maturity of pancreatic stem cells (PSCs) due to its similarity to the pancreas. To determine the effect of the liver niche on the differentiation of PSCs, we used neonatal Wistar rat pancreata for cultivation of PSCs, which were transplanted into diabetic Wistar rats via the portal vein or beneath the renal capsule. After transplantation, we measured random blood glucose, weight, and serum insulin and performed an intraperitoneal glucose tolerance test. Specimens were examined by immunofluorescence. As a result, highly proliferating harvested cells showed the characteristics of stem cells. The PSCs could be induced to form large islet-like structures (150–200 μm diameter) in the liver, which resulted in better therapeutic efficacy. In contrast, there were smaller islet-like structures (about 50 μm diameter) when PSCs were transplanted beneath the renal capsule. These findings demonstrated that the liver niche benefits the in vivo differentiation of PSCs into endocrine and exocrine cells that may contribute to the generation of insulin producing cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Transplantation Proceedings - Volume 41, Issue 9, November 2009, Pages 3898–3904
نویسندگان
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