کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4262026 1284601 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Irradiation Is an Early Determinant of Endothelial Injury During Hematopoietic Stem Cell Transplantation
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
پیش نمایش صفحه اول مقاله
Irradiation Is an Early Determinant of Endothelial Injury During Hematopoietic Stem Cell Transplantation
چکیده انگلیسی

ObjectiveWe investigated the degree and the time course of endothelial injury in mice pretreated with lethal or reduced-intensity irradiation administered before transplantation.Materials and MethodsSix- to eight-week-old female mice were randomly allocated into three groups: lethal-intensity irradiation (8.5 Gy, group 1), reduced-intensity irradiation (5.0 Gy, group 2), or nonirradiated controls (group 3). After conditioning, circulating endothelial cells (CD31+, CD133−, and CD45low) and peripheral blood CD4+ or CD8+ T-lymphocyte subpopulations were enumerated using flow cytometry at various times. The morphologic changes in endothelium were examined at phase-contrast light microscopy.ResultsCirculating endothelial cells showed an earlier and higher peak in the lethal irradiation group compared with the reduced-intensity irradiation group, which exhibited a protean elevation in cell numbers. There were no visible histopathologic changes during the early stage of endothelial damage.ConclusionsLethal and reduced doses of irradiation induced endothelial injury in a dose-dependent manner. Endothelial damage may occur before graft-vs-host disease and its related complications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Transplantation Proceedings - Volume 40, Issue 8, October 2008, Pages 2661–2664
نویسندگان
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