کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4280972 | 1611567 | 2009 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Surgical experience of refined 3-cuff technique for orthotopic small-bowel transplantation in rat: a report of 270 cases Surgical experience of refined 3-cuff technique for orthotopic small-bowel transplantation in rat: a report of 270 cases](/preview/png/4280972.png)
BackgroundEstablishment of an instant, reproducible, and reliable rat model of a refined 3-cuff technique for performing orthotopic intestinal transplantation is reported, and the surgical skills required to perform modified surgical procedure are discussed.MethodsA retrospective analysis was used to study 270 rat cases subject to orthotopic intestinal transplantation (OIT) performed in our transplantation center from March 2006 to March 2008. After establishing the portal vein cuff method, a conventional hand-sewn anastomosis method combination, with porto-to-portal re-establishment by cuffed anastomosis technique, was used in group 1 (n = 140), and the modified 3-cuff anastomosis method was applied in group 2 (n = 130). Statistical comparison was made between the 2 groups.ResultsIn group 1, 97 of 140 (69.3%) recipients survived >7 days, and 69 (49.3%) survived >30 days, whereas in group 2, respective survival was 110 of 130 (84.6%) and 86 of 130 (66.2%). Average cold ischemic times in the 2 groups were 48.5 ± 5.1 minutes and 31 ± 3.0 minutes, respectively. There was a significant difference between the 2 groups (P <.05). In most cases, the average volume of bleeding during recipient surgery was <1 mL using the simplified 3-cuff anastomosis technique. There was shorter graft revascularization time with the new model of sutureless microanastomosis using cuff apparatus for OIT in rats compared with the control group. The method adopted in group 2 was much easier, more stable, and more feasible than that in group 1. Sixty-three rats died in 7 days, and autopsy verified the causative factors leading to death, which are summarized in the text. The results obtained were acceptable and satisfactory. Overall, there was a comparative lower incidence of complications associated with the procedure used in group 2.ConclusionsThe modified 3-cuff anastomosis technique for rat OIT models has several obvious advantages, which can be summarized as follows: vascular anastomosis is stable and simplified, and blood loss is significantly decreased; natural anatomic physiologic portal graft drainage is maintained; and intraoperative mortality and postsurgical morbidity are minimized. Furthermore, technical refinement of rat OIT models established by our research team can be carried out without a microscope and can be easily implemented in the laboratory by 1 trainee with acceptable success after a short period of training. We regard it as one of the best available orthotopic small-bowel transplantation methods in rat.
Journal: The American Journal of Surgery - Volume 198, Issue 1, July 2009, Pages 110–121