کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4281968 1611587 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Morphologic characterization of early ligation–induced acute pancreatitis in rats
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
پیش نمایش صفحه اول مقاله
Morphologic characterization of early ligation–induced acute pancreatitis in rats
چکیده انگلیسی

BackgroundBile-pancreatic duct ligation in rats causes acute pancreatic inflammation. We performed serial morphologic evaluation of the exocrine pancreas after duct ligation to facilitate further investigations using the model.MethodsThe pancreas was excised from 74 rats after 0, 1, 3, 5, 24 or 48 hours of duct ligation or sham surgery. A pathologist evaluated 1 hematoxylin- and eosin-stained slide from each rat. Confirmatory immunostaining was performed with markers for apoptosis (activated caspase-3), proliferation (cyclin D3), neutrophils (myeloperoxidase), and macrophages (CD68).ResultsInterstitial edema and white blood cell infiltration were apparent at 24 hours and increased at 48 hours. Progressive periods of duct ligation were characterized by ductular ectasia (1 to 3 hours), acinar vacuolization (5 to 48 hours), leukocytic margination and neutrophil exocytosis (5 to 48 hours), ductule epithelium hypertrophy and proliferation (24 to 48 hours), and discernible loss of zymogen granules (48 hours).ConclusionsLigation-induced acute pancreatitis in rats is a useful model to investigate early events in disease pathogenesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The American Journal of Surgery - Volume 194, Issue 5, November 2007, Pages 652–658
نویسندگان
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