کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4299470 1288392 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A murine model of lung ischemia and reperfusion injury: tricks of the trade
ترجمه فارسی عنوان
یک مدل موشکی از ایسکمی ریه و آسیب مجدد ریه: ترفندهای تجارت
کلمات کلیدی
ایسکمی ریوی رپرفیوژن، آسیب مجدد ایسکمی-مجدد ماوس، مدل تجربی
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
چکیده انگلیسی

BackgroundPulmonary ischemia–reperfusion injury (IRI) causes postoperative morbidity in patients undergoing lung transplantation, isolated lung perfusion, and cardiopulmonary bypass and may lead to potentially lethal pathologies such as respiratory shock. In-depth study of this pathology requires a reliable animal model. Mice are a popular species to develop experimental models because of their logistic advantages and the availability of knock outs. However, their small size warrants microsurgical techniques and a skilled surgeon.Materials and methodsWe developed a murine model of pulmonary anoxic IRI through hilar clamping using adult female Swiss mice. After left thoracotomy, we expose the pulmonary hilum keeping the ribs and the muscles of back and forepaw intact. A microvascular clamp is placed over the entire hilum, occluding bronchus, pulmonary artery, and vein.ResultsOur model proved to be simple, reliable, and reproducible, showing minimal preoperative and postoperative mortality. Histopathologic analysis indicated all characteristic features of pulmonary IRI, such as an early recruitment of lymphocytes followed by neutrophil influx.ConclusionsThis article presents a murine surgery model for pulmonary IRI based on a muscle-sparing thoracotomy. The minimal approach limits manipulation of lung tissue, minimizing mortality and non–IRI-induced injury.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Surgical Research - Volume 194, Issue 2, April 2015, Pages 659–666
نویسندگان
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