کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4300019 1288408 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Establishment of a bilateral femoral large segmental bone defect mouse model potentially applicable to basic research in bone tissue engineering
ترجمه فارسی عنوان
ایجاد مدل موش ناقص استخوانی بزرگ فمورال بزرگ به طور بالقوه برای تحقیقات اولیه در مهندسی بافت استخوانی کاربرد دارد
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
چکیده انگلیسی

BackgroundTo understand the cellular mechanism underlying bone defect healing in the context of tissue engineering, a reliable, reproducible, and standardized load-bearing large segmental bone defect model in small animals is indispensable. The aim of this study was to establish and evaluate a bilateral femoral defect model in mice.Materials and methodsDonor mouse bone marrow mesenchymal stem cells (mBMSCs) were obtained from six mice (FVB/N) and incorporated into partially demineralized bone matrix scaffolds to construct tissue-engineered bones. In total, 36 GFP+ mice were used for modeling. Titanium fixation plates with locking steel wires were attached to the femurs for stabilization, and 2-mm–long segmental bone defects were created in the bilateral femoral midshafts. The defects in the left and right femurs were transplanted with tissue-engineered bones and control scaffolds, respectively. The healing process was monitored by x-ray radiography, microcomputed tomography, and histology. The capacity of the transplanted mBMSCs to recruit host CD31+ cells was investigated by immunofluorescence and real-time polymerase chain reaction.ResultsPostoperatively, no complication was observed, except that two mice died of unknown causes. Stable fixation of femurs and implants with full load bearing was achieved in all animals. The process of bone defect repair was significantly accelerated due to the introduction of mBMSCs. Moreover, the transplanted mBMSCs attracted more host CD31+ endothelial progenitors into the grafts.ConclusionsThe present study established a feasible, reproducible, and clinically relevant bilateral femoral large segmental bone defect mouse model. This model is potentially suitable for basic research in the field of bone tissue engineering.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Surgical Research - Volume 192, Issue 2, December 2014, Pages 454–463
نویسندگان
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