کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4300411 1288420 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Postconditioning of the small intestine: which is the most effective algorithm in a rat model?
ترجمه فارسی عنوان
تهویه مطبوع روده کوچک: کدام یک از الگوریتم های موثر در یک مدل موش است؟
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
چکیده انگلیسی

BackgroundMesenteric ischemia is a serious clinical condition requiring immediate surgical intervention. The unavoidable ischemic-reperfusion (IR) injury may be ameliorated using the appropriate postconditioning protocol. The aim of the present study was to investigate the optimal postconditioning algorithm in a rat model of intestinal ischemic–reperfusion injury.Materials and methodsMale Wistar rats were randomized into five groups (n = 10), one sham-operated, one IR, and three postconditioned groups, each with different protocols. The animals were subjected to 60 min of mesenteric ischemia, followed by 60 min of reperfusion. Postconditioning was applied at the onset of reperfusion using three different algorithms. Arterial pressure and mucosal microcirculation were monitored throughout the experiment. Mesenteric pH was determined at the early phase of reperfusion. Blood and tissue samples were taken at the end of reperfusion for histologic evaluation, serum lactate dehydrogenase, serum creatine kinase, serum tumor necrosis factor-α, serum interleukin-6, detailed mucosal antioxidant, and scavenger capacity assays.ResultsThe shorter and intermediate length cycles of postconditioning enhanced mucosal microcirculation and redox state and significantly delayed the normalization of mesenteric pH. Furthermore, milder histopathologic lesions and lower concentrations of serum necroenzymes and proinflammatory cytokines were detected compared with the IR group. The protective effect of postconditioning using longer cycles could only be seen in a tendentious manner.ConclusionsIn a rat model of intestinal ischemia–reperfusion, the shorter and intermediate length cycles of postconditioning proved to be more effective than the use of longer cycles.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Surgical Research - Volume 187, Issue 2, April 2014, Pages 427–437
نویسندگان
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