کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4301081 1288431 2013 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Induction of therapeutic neoangiogenesis using In vitro–generated endothelial colony-forming cells: an autologous transplantation model in rat
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
پیش نمایش صفحه اول مقاله
Induction of therapeutic neoangiogenesis using In vitro–generated endothelial colony-forming cells: an autologous transplantation model in rat
چکیده انگلیسی

BackgroundAccumulating evidence shows the potential of bone marrow–derived endothelial colony-forming cells (bmECFCs) as promising tools for vascular repair. However, knowledge about their in vitro expansion, characterization, and functional behavior is still controversial. We demonstrate the in vitro generation of rat bmECFCs and analyze their ability to promote tissue reperfusion in a chronic hind-limb ischemia model.MethodsEither in vitro–generated and characterized autologous bmECFCs or placebo was injected into ischemic hind limbs of Sprague-Dawley rats. Tissue perfusion was quantified by laser Doppler, in perfusion units (PU), at days 0, 15, and 30.ResultsRat bmECFCs acquire a typical phenotype (CD34+VEGFR2+CD133+CXCR4+CD45−), culture, and functional behavior (Dil-ac-LDL+) in vitro. Injection of autologous bmECFCs improves tissue perfusion in ischemic hind limbs (183.5 ± 3.29 PUbmECFCs/day 30versus 131 ± 3.9 PUcontrols/day 30, P < 0.001).ConclusionsWe conclude that rat bmECFCs promote ischemic tissue reperfusion and their proangiogenic properties are a potential mechanism for this effect.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Surgical Research - Volume 181, Issue 2, 15 May 2013, Pages 359–368
نویسندگان
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