کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4301454 | 1288438 | 2012 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Phenacetin O-Deethylation is a Useful Tool for Evaluation of Hepatic Functional Reserve in Rats with CCl4-Induced Chronic Liver Injury Phenacetin O-Deethylation is a Useful Tool for Evaluation of Hepatic Functional Reserve in Rats with CCl4-Induced Chronic Liver Injury](/preview/png/4301454.png)
BackgroundMortality of liver resection is as high as 3.1% to 25% in patients with chronic liver disease. Evaluation of hepatic functional reserve is critical for the prediction of risk of postoperation death. Phenacetin O-deethylation is a marker reaction of cytochrome P4501A2 (CYP1A2) activity. In this study, our aim is to investigate whether phenacetin O-deethylation is a useful tool for the evaluation of hepatic functional reserve in rats with chronic liver injury.Materials and MethodsRat model for chronic liver injury was established by subcutaneous administration of 50% CCl4, 1 mL/kg twice per week for 12 wk. Hepatic CYP1A2 activity, content, and mRNA expression were determined (n = 10). Effects of 15%, 30%, and 45% hepatectomy on phenacetin O-deethylation were evaluated in the rats (n = 5 in each group). Additionally, the correlation of risk of death after 70% hepatectomy with phenacetin O-deethylation was studied in 27 rats with chronic liver injury.ResultsCompared with normal controls, CYP1A2 activity, content, and mRNA expression decreased 33%, 60%, and 50% in the rats with chronic liver injury (P < 0.05), respectively. Following the increasing of liver-resected size, CYP1A2 activity decreased proportionally (rs = −0.877, P < 0.05). Six of 27 rats with chronic liver injury died within 7 d after 70% hepatectomy. Phenacetin metabolism was impaired more severely in 6 rats that died than in 21 living rats (P < 0.05).ConclusionsPhenacetin O-deethylation is a useful tool for the evaluation of hepatic functional reserve in the rats with CCl4-induced chronic liver injury.
Journal: Journal of Surgical Research - Volume 175, Issue 2, 15 June 2012, Pages e61–e66