کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4301869 1288444 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In Utero Gene Delivery Using Chitosan-DNA Nanoparticles in Mice
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
پیش نمایش صفحه اول مقاله
In Utero Gene Delivery Using Chitosan-DNA Nanoparticles in Mice
چکیده انگلیسی

BackgroundIn utero gene transfer is a novel therapy for monogenic disorders diagnosed in the fetus. Enhanced biosafety alternatives to viral vectors include non-viral transfer agents such as chitosan. The purpose of this study was to evaluate in vitro and in utero gene transfer of reporter gene (GFP) using chitosan as a transfer vehicle.Materials and MethodsIn vitro studies: 1. Chitosan colloidal suspensions were prepared, and particle stability in murine amniotic fluid (AF) was determined. 2. Chitosan-reporter gene (EGFP) constructs were prepared and protection from endogenous digestion in AF was measured by gel electrophoresis. 3. Transfection efficiency (by chitosan-EGFP) of HEK293T cells was determined in varying proportions of medium and AF. In utero studies: Amniotic sacs of time-mated CD-1 mice were injected with chitosan-pEGFP (12.5 μg DNA) on G17. Pups and their dams were sacrificed and tissues were examined for transgene presence and expression.ResultsChitosan formed stable aggregates in AF. Although AF decreased in vitro transfection efficiency, in vivo transfection by amniotic injection achieved short-term transgene expression in pup lung and intestine.ConclusionsIn utero delivery of chitosan-EGFP results in postnatal gene expression, and shows promise for non-viral gene transfer in animal models of fetal gene therapy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Surgical Research - Volume 171, Issue 2, December 2011, Pages 691–699
نویسندگان
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