کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4303356 1612531 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Complement Component C5a Mediates Hemorrhage-Induced Intestinal Damage
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
پیش نمایش صفحه اول مقاله
Complement Component C5a Mediates Hemorrhage-Induced Intestinal Damage
چکیده انگلیسی

BackgroundComplement has been implicated in the pathogenesis of intestinal damage and inflammation in multiple animal models. Although the exact mechanism is unknown, inhibition of complement prevents hemodynamic alterations in hemorrhage.Materials and methodsC57Bl/6, complement 5 deficient (C5–/–) and sufficient (C5+/+) mice were subjected to 25% blood loss. In some cases, C57Bl/6 mice were treated with C5a receptor antagonist (C5aRa) post-hemorrhage. Intestinal injury, leukotriene B4, and myeloperoxidase production were assessed for each treatment group of mice.ResultsMice subjected to significant blood loss without major trauma develop intestinal inflammation and tissue damage within 2 hours. We report here that complement 5 (C5) deficient mice are protected from intestinal tissue damage when subjected to hemorrhage (injury score = 0.36 compared with wildtype hemorrhaged animal injury score = 2.89; P < 0.05). We present evidence that C5a represents the effector molecule because C57Bl/6 mice treated with a C5a receptor antagonist displayed limited intestinal injury (injury score = 0.88), leukotriene B4 (13.16 pg/mg tissue), and myeloperoxidase (115.6 pg/mg tissue) production compared with hemorrhaged C57Bl/6 mice (P < 0.05).ConclusionsComplement activation is important in the development of hemorrhage-induced tissue injury and C5a generation is critical for tissue inflammation and damage. Thus, therapeutics targeting C5a may be useful therapeutics for hemorrhage-associated injury.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Surgical Research - Volume 150, Issue 2, December 2008, Pages 196–203
نویسندگان
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