کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4303501 | 1288480 | 2009 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Intestinal Edema: Effect of Enteral Feeding on Motility and Gene Expression Intestinal Edema: Effect of Enteral Feeding on Motility and Gene Expression](/preview/png/4303501.png)
ObjectiveEdema formation, inflammation, and ileus in the intestine are commonly seen in conditions like gastroschisis, inflammatory bowel disease, and cirrhosis. We hypothesized that early enteral feeding would improve intestinal transit. We also wanted to study the impact of early enteral feeding on global gene expression in the intestine.DesignRats were divided into Sham or Edema ± immediate enteral nutrition (IEN). At 12 h, small intestinal transit via FITC-Dextran and tissue water were measured. Ileum was harvested for total RNA to analyze gene expression using cDNA microarray with validation using real-time PCR. Data are expressed as mean ± SEM, n = 4–6 and ∗, ∗∗ = P < 0.05 versus all groups using ANOVA.ResultsIEN markedly improved intestinal transit with minimal genetic alterations in Edema animals. Major alterations in gene expression were detected in primary, cellular and macromolecular metabolic activities. Edema also altered more genes involved with the regulation of the actin cytoskeleton.ConclusionsIntestinal edema results in impaired small intestinal transit and globally increased gene expression. Early enteral nutrition improves edema-induced impaired transit and minimizes gene transcriptional activity.
Journal: Journal of Surgical Research - Volume 155, Issue 2, August 2009, Pages 283–292