Differential Expression of Procollagen Genes Between Mid- and Late-Gestational Fetal Fibroblasts

Our results from this in vitro work demonstrate a differential pattern of gene expression for procollagen 1α1 and 3 in E15 and E18 fibroblasts in response to TGF-β1. E15 cells showed decreased expression of procollagen 1α1, while E18 cells showed increased procollagen 1α1 and decreased procollagen 3 expression. These patterns of expression in E15 cells are suggestive of increased type 3 to 1 collagen ratio seen in scarless fetal wounds. Interestingly, treatment of either E15 or E18 cells with TGF-β1 significantly decreased procollagen 3 expression by 48 h, yet this was more profound in E15 groups. This suggests that after 24 h, E15 cells may transition towards an E18 phenotype and corresponding signaling.