کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4304635 1288510 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Normal Mesenteric Lymph Blunts the Pulmonary Inflammatory Response to Endotoxin
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
پیش نمایش صفحه اول مقاله
Normal Mesenteric Lymph Blunts the Pulmonary Inflammatory Response to Endotoxin
چکیده انگلیسی

BackgroundMesenteric lymph may provide the mechanistic link between gut ischemia and acute lung injury after hemorrhagic shock (HS). Studies have focused on the toxic mediators that develop in the post-shock mesenteric lymph (PSML). However, a complementary possibility is that there is loss of protective mediators found in pre-shock normal mesenteric lymph (NML) after HS. We hypothesize that NML protects against inflammatory insults to the pulmonary endothelium and that this effect is lost in PSML.Materials and methodsPrimary human pulmonary endothelial cells (HMVECs) were incubated with NML or PSML collected from rats subjected to HS and resuscitation and then stimulated with 20 ng/mL LPS. ICAM-1 surface expression was measured by flow cytometry. In subsequent experiments, lipoproteins were extracted from NML before incubation and LPS-induced ICAM-1 expression determined.ResultsMean fluorescent intensity (MFI) of LPS-induced ICAM-1 in NML and PSML treated HMVECs were 10.1 ± 2.3 versus 27.7 ± 0.83, respectively (P < 0.05). This represented at 71% decrease in ICAM-1 expression by NML compared to ICAM-1 expression in LPS-induced controls (MFI: 34.6 ± 6.9). Lipoprotein extraction from NML abolished this protective effect (MFI: 31.2 ± 5.3 versus Control + LPS: 33.5 ± 3.6, P > 0.05). Baseline ICAM-1 levels were not significantly different among control, NML, and PSML groups.ConclusionLipoproteins in NML contain anti-inflammatory properties that decrease ICAM-1 expression induced by LPS in pulmonary endothelium. Decreased protective lipoproteins after HS and resuscitation may contribute to the toxicity associated with PSML from the ischemic gut.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Surgical Research - Volume 136, Issue 2, December 2006, Pages 166–171
نویسندگان
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