کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4308766 | 1289290 | 2010 | 14 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Peritoneal adhesion formation and reformation tracked by sequential laparoscopy: Optimizing the time point for adhesiolysis Peritoneal adhesion formation and reformation tracked by sequential laparoscopy: Optimizing the time point for adhesiolysis](/preview/png/4308766.png)
BackgroundIn a high proportion of patients, operatively lysed adhesions reform. Using a rabbit adhesiogenesis model, this study assessed the efficacy of adhesiolysis and examined how this relates to the tissue composition of adhesions at the time of lysis.MethodsPolypropylene meshes (5 × 3.5 cm) were implanted on the parietal peritoneum of New Zealand white rabbits. Some animals were killed 3, 7, 14, and 90 days postimplantation to obtain adhesion tissue. Adhesion formation/reformation was monitored by sequential laparoscopy in other animals kept for 90 days and in a separate experimental group subjected to adhesiolysis at 3 days postimplantation. Immune and inflammatory response markers were determined by immunohistochemical, Western blotting, and real-time reverse transcriptase polymerase chain reaction procedures in adhesion tissue; areas occupied by adhesions were quantified in meshes.ResultsIn animals undergoing adhesiolysis, mesh areas covered by adhesions were significantly decreased at each follow-up time and affected areas became mesothelialized. Increased transforming growth factor (TGF)-β1 expression was detected in adhesions at 3 days. Greatest TGF-β1 and vascular endothelial growth factor (VEGF) protein expressions were observed at 7 days, whereas genetic overexpression was noted at 14 days. Active inflammatory cells peaked at the 7-day time point.ConclusionAdhesions formed at 3 days; at this critical time, an adhesiolysis was effective in preventing reformation of future adhesions. TGF-β1 gene and protein expression were increased in 3-day adhesions with respect to the omentum. Levels of active TGF-β1 and VEGF were increased at 7 days, along with the inflammatory response at this time point related to tissue remodeling, which led to stabilization of adhesions.
Journal: Surgery - Volume 147, Issue 3, March 2010, Pages 378–391