Inactivation of the prelimbic cortex enhances freezing induced by trimethylthiazoline, a component of fox feces

► Determine effect of mPFC inactivation on unconditioned defense behavior. ► Prelimbic, but not infralimbic, inactivation enhances unconditioned freezing. ► Neither prelimbic nor infralimbic inactivation alters open space avoidance. ► Prelimbic cortex can have inhibitory role with regard to unconditioned freezing.