کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4318903 1613261 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
MiR-21 involve in ERK-mediated upregulation of MMP9 in the rat hippocampus following cerebral ischemia
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
MiR-21 involve in ERK-mediated upregulation of MMP9 in the rat hippocampus following cerebral ischemia
چکیده انگلیسی

Matrix metallinoprotease-9 (MMP9) plays a key role in the pathogenesis of post-ischemic blood brain barrier (BBB) disruption and the formation of lesions after cerebral ischemia. In this study we investigate the effect of brain-specific miRNAs on MMP-9 protein level in the rat hippocampus following cerebral ischemia and its underlying mechanism. Cerebral ischemia significantly upregulated miR-21 and -224 in the hippocampus; however, expression of miR-122 and -338-3p was not significantly affected by ischemia. Silencing of miR-21, but not -224, reduced MMP9 protein level after cerebral ischemia. Downregulation of extracellular signal-regulated kinase (ERK) signaling using the ERK inhibitor U0126 and the calcium-channel blocker ketamine inhibited the upregulation of miR-21 expression and MMP9 protein level after cerebral ischemia. The study suggests that cerebral ischemia up-regulates expression level of miR-21, which is involved in ERK-stimulated upregulation of MMP9 following cerebral ischemia via a calcium-dependent mechanism.


► Cerebral ischemia induces significant up-regulation of miR-21 and -224.
► Gene silence of miR-21 but not -224 can reduce increment of MMP9 protein levels.
► MiR-21 plays an important role in ERK-induced MMP9 expression after ischemia.
► MiR-21 mediated MMP9 up-regulation is closely linked with calcium signal.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research Bulletin - Volume 94, May 2013, Pages 56–62
نویسندگان
, , , , ,