کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4320052 | 1290845 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Natriuretic peptide receptor-A is functionally expressed on bullfrog retinal Müller cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
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چکیده انگلیسی
By the patch clamp technique, whole-cell currents induced by brain natriuretic peptide (BNP) from isolated bullfrog retinal Müller cells were studied. Application of 100Â nM BNP induced a sustained inward current from these cells with a reversal potential of about 0Â mV, and the current could be completely blocked by anantin, an antagonist of the A-type NP receptor (NPR-A) and CdCl2, a blocker of cyclic nucleotide-gated (CNG) non-selective cation channels. Likewise, perfusion with the membrane-permeable cGMP analog 8-bromoguanosine-3â²,5â²-cyclic monophosphate (8Br-cGMP) caused effects that are similar to those of BNP. Moreover, application of BNP failed to induce any current in the presence of 1Â mM 8Br-cGMP. By calcium imaging, we further showed a significant increase in intracellular calcium levels ([Ca2+]i) of all parts of Müller cells, including the endfoot, soma and processes following the perfusion of BNP, and the increase could be blocked by anantin. All these results suggest that NPR-A is expressed in bullfrog Müller cells, and activation of the receptor causes an increase of intracellular cGMP levels that activates CNG channels and thereby results in an increased calcium influx.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research Bulletin - Volume 71, Issue 4, 9 January 2007, Pages 410-415
Journal: Brain Research Bulletin - Volume 71, Issue 4, 9 January 2007, Pages 410-415
نویسندگان
Li-Hui Cao, Xiong-Li Yang,