کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4323906 1613833 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Allosteric modulation of the glycine receptor activated by agonists differing in efficacy
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Allosteric modulation of the glycine receptor activated by agonists differing in efficacy
چکیده انگلیسی


• Glycine and taurine are agonists that vary in their efficacies at the GlyR.
• GlyR function is affected by allosteric modulators such as zinc and ethanol.
• Zinc enhancement was greater for taurine-activated than glycine-activated receptors.
• The degree of allosteric modulation depends on the efficacy of the agonist tested.

The glycine receptor (GlyR) is the predominant inhibitory neurotransmitter receptor in the brainstem and spinal cord but is also found in higher brain regions. GlyR function is affected by a variety of allosteric modulators including drugs of abuse, such as ethanol and inhalants and the ubiquitous divalent cation zinc. Two-electrode voltage-clamp experiments were conducted on Xenopus laevis oocytes expressing wild-type α1 homomeric glycine receptors to compare the degree of enhancement produced by zinc on GlyR activated by two agonists (glycine vs. taurine) that vary markedly in their efficacies. Zinc potentiation of both glycine- and taurine-evoked currents was the same at the concentrations of agonists that produced the same currents, corresponding to 6% of the maximal effect of glycine compared to 23% of the maximal effect of taurine. Similar results were seen with 50 and 200 mM ethanol. A direct comparison of agonist concentration–response curves showed that zinc enhancement was greater, overall, for taurine-activated than glycine-activated receptors. In addition, zinc only enhanced taurine- but not glycine-activated GlyR when agonists were applied at saturating concentrations. These data suggest that zinc affects taurine affinity, as well as the probability of channel opening at sub-maximal taurine concentrations, and that the magnitude of allosteric modulation at the GlyR depends on the efficacy of the agonist tested. This has implications for mutagenesis studies in which changes in the degree of allosteric modulation observed may result from mutation-induced changes in agonist efficacy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1606, 5 May 2015, Pages 95–101
نویسندگان
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