کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4324029 1613845 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Norepinephrine transporter knock-out alters expression of the genes connected with antidepressant drugs action
ترجمه فارسی عنوان
غربالگری نوراپی نفرین باعث تغییر بیان ژن های متصل به داروهای ضد افسردگی می شود
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


• Genes linked with antidepressant treatment are altered in the brain of NET-KO mice.
• Expression of neuropeptide and neurotrophin coding genes is higher in NET-KO brain.
• Crh-bp mRNA expression is increased in the cortex and the hippocampus of NET-KO mice.

Norepinephrine transporter knock-out mice (NET-KO) exhibit depression-resistant phenotypes. They manifest significantly shorter immobility times in both the forced swim test and the tail suspension test. Moreover, biochemical studies have revealed the up-regulation of other monoamine transporters (dopamine and serotonin) in the brains of NET-KO mice, similar to the phenomenon observed after the chronic pharmacological blockade of norepinephrine transporter by desipramine in wild-type (WT) animals. NET-KO mice are also resistant to stress, as we demonstrated previously by measuring plasma corticosterone concentration. In the present study, we used a microdissection technique to separate target brain regions and the TaqMan Low Density Array approach to test the expression of a group of genes in the NET-KO mice compared with WT animals. A group of genes with altered expression were identified in four brain structures (frontal and cingulate cortices, dentate gyrus of hippocampus and basal-lateral amygdala) of NET-KO mice compared with WT mice. These genes are known to be altered by antidepressant drugs administration. The most interesting gene is Crh-bp, which modulates the activity of corticotrophin – releasing hormone (CRH) and several CRH-family members. Generally, genetic disturbances within noradrenergic neurons result in biological changes, such as in signal transduction and intercellular communication, and may be linked to changes in noradrenaline levels in the brains of NET-KO mice.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1594, 12 January 2015, Pages 284–292
نویسندگان
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