کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4325326 1613993 2012 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Changes in glycine receptor subunit expression in forebrain regions of the Wistar rat over development
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Changes in glycine receptor subunit expression in forebrain regions of the Wistar rat over development
چکیده انگلیسی

Glycine receptors (GlyRs) are pentameric membrane proteins in the form of either α-homomers or α–β heteromers. Four out of five subunits; α1–3 and β, have been found in the mammalian brain. Early studies investigating subunit composition and expression patterns of this receptor have proposed a developmental switch from α2 homomers to α1β heteromers as the CNS matures, a conclusion primarily based on results from the spinal cord. However, our previous results indicate that this might not apply to e.g. the forebrain regions. Here we examined alterations in GlyR expression caused by developmental changes in selected brain areas, focusing on reward-related regions. Animals of several ages (P2, P21 and P60) were included to examine potential changes over time. In accordance with previous reports, a switch in expression was observed in the spinal cord. However, the present results indicate that a decrease in α2 subunit expression is not replaced by α1 subunit expression since the generally low levels, and modest increases, of α1 could hardly replace the reduction in α2-mRNA. Instead mRNA measurements indicate that α2 continues to be the dominating α-subunit also in adult animals, usually in combination with high and stable levels of β-subunit expression. This indicates that alterations in GlyR subunit expression are not simply a maturation effect common for the entire CNS, but rather a unique pattern of transition depending on the region at hand.


► There is no general switch in expression from α2 to α1β in the forebrain regions.
► The pattern of GlyR subunit expression varies depending on the brain region studied.
► α2 is the most highly expressed α-subunit at all ages, and in all regions, but the SC.
► IHC show that GlyR-positive cells in the nAc are situated primarily in the shell.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1446, 29 March 2012, Pages 12–21
نویسندگان
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