کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4326358 | 1614077 | 2010 | 9 صفحه PDF | دانلود رایگان |
During development, the rescue of spinal motoneurons as well as sensory neurons in the dorsal root ganglion (DRG) from programmed cell death (PCD) depends on the integrity of peripheral target innervation. Following deletion of the pro-apoptotic gene Bax, both motoneurons and DRG neurons are rescued from PCD. In the present paper, we asked whether different cell types in the DRG exhibit distinct responses to Bax deletion. In 1-month-old Bax-deficient (Bax−/−) mice, distinct subsets of DRG neurons that were immunopositive for TrkA, CGRP, TRPV1 or TrkC, were all increased in number and exhibited cell atrophy compared to wild type DRG neurons. In addition there was hyperinnervation of the epidermis by CGRP immunopositive processes and a correlated functional hypersensitivity of mechanical nociception in Bax−/− mice. By contrast, the functional properties of populations of rescued thermoreceptor and mechanoreceptor DRG neurons were unchanged. These data indicate that although Bax deletion rescues all of the DRG cell types examined here from PCD, the functional consequences of having excess cells differ between sensory phenotypes.
Research Highlights
► Bax deletion rescued all subtypes of DRG neurons from programmed cell death.
► The excess DRG neurons exhibited decreased soma size following Bax deletion.
► The only CGRP positive neurons showed skin hyperinnervation in Bax-deficient mice.
► The functional consequences of having excess DRG neurons differ between subtypes.
Journal: Brain Research - Volume 1362, 29 November 2010, Pages 23–31