کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4326488 1614084 2010 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Co-localization of TRHR1 and LepRb receptors on neurons in the hindbrain of the rat
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Co-localization of TRHR1 and LepRb receptors on neurons in the hindbrain of the rat
چکیده انگلیسی

We have reported a highly cooperative interaction between leptin and thyrotropin releasing hormone (TRH) in the hindbrain to generate thermogenic responses (Hermann et al., 2006) (Rogers et al., 2009). Identifying the locus in the hindbrain where leptin and TRH act synergistically to increase thermogenesis will be necessary before we can determine the mechanism(s) by which this interaction occurs. Here, we performed heat-induced epitope recovery techniques and in situ hybridization to determine if neurons or afferent fibers in the hindbrain possess both TRH type 1 receptor and long-form leptin receptor [TRHR1; LepRb, respectively]. LepRb receptors were highly expressed in the solitary nucleus [NST], dorsal motor nucleus of the vagus [DMN] and catecholaminergic neurons of the ventrolateral medulla [VLM]. All neurons that contained LepRb also contained TRHR1. Fibers in the NST and the raphe pallidus [RP] and obscurrus [RO] that possess LepRb receptors were phenotypically identified as glutamatergic type 2 fibers (vglut2). Fibers in the NST and RP that possess TRHR1 receptors were phenotypically identified as serotonergic [i.e., immunopositive for the serotonin transporter; SERT]. Co-localization of LepRb and TRHR1 was not observed on individual fibers in the hindbrain but these two fiber types co-mingle in these nuclei. These anatomical arrangements may provide a basis for the synergy between leptin and TRH to increase thermogenesis.

Research highlights
► Leptin acts to “gate” thermogenic effects of TRH.
► These gating effects occur in the hindbrain.
► Leptin and TRH receptors are located on same neurons in the brainstem.
► Fourth ventricular leptin activates pSTAT3 only in neurons in solitary nucleus.
► NST is critical for leptin control of thermogenesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1355, 8 October 2010, Pages 70–85
نویسندگان
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