کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4326627 1614088 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Steroidal and gonadal effects on neural cell proliferation in vitro in an adult songbird
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Steroidal and gonadal effects on neural cell proliferation in vitro in an adult songbird
چکیده انگلیسی

Neurogenesis in the adult songbird brain occurs along the ventricular zone (VZ), a specialized cell layer surrounding the lateral ventricles. To examine the acute effects of sex steroids on VZ cell proliferation, male and female adult zebra finch brain slices containing the VZ were exposed to 5-bromo-2′-deoxyuridine-5′-monophosphate (BrdU) in vitro. Slices from one hemisphere served as the control, while contralateral slices were treated with steroids, steroidogenic enzyme inhibitors or gonadal tissue itself. There were no significant effects on VZ cell proliferation in either sexes by acute exposure to 17β-estradiol (E2), dihydrotestosterone (DHT), a cocktail of four sex steroids, and inhibitors of sex steroid synthesis (aminoglutethimide, ketoconazole, and fadrozole), or by activation of a mitochondrial cholesterol transporter. By contrast, dehydroepiandrosterone (DHEA) suppressed VZ cell proliferation in males, but not females, replicating previous observations involving treatments with corticosterone and RU-486. This suggests that DHEA suppresses proliferation in males via a glucocorticoid receptor-related mechanism. These results suggest that neurosteroidogenesis per se has little effect on acute VZ cell proliferation. Co-incubation with an ovary of female, but not male, slices significantly increased VZ cell proliferation; testicular tissue had no impact on proliferation in males or females. This suggests a role for a non-steroidal ovarian factor on adult female VZ cell proliferation. We also have evidence that previously reported sex-differences in BrdU-labeling along the adult VZ (males > females) result from a more rapid loss of cells in females. Sex differences in steroid action and cell death along the VZ may contribute to the maintenance of the sexually dimorphic song system.

Research highlights
► Steroidal and gonadal effects on VZ cell proliferation in adult zebra finch brain.
► Sex steroids do not affect VZ cell proliferation in adult zebra finch brain.
► DHEA reduces VZ cell proliferation only in adult male zebra finches.
► Ovaries increase VZ cell proliferation in adult female zebra finches.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1351, 10 September 2010, Pages 41–49
نویسندگان
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