کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4328053 1614159 2009 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Activity-dependent neuronal control of gap-junctional communication in fibroblasts
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Activity-dependent neuronal control of gap-junctional communication in fibroblasts
چکیده انگلیسی

Intercellular communication through gap junctions plays an important role in fibroblast physiology. Here we report an activity-dependent neuronal control of gap-junctional communication (GJC) in rat dermal fibroblasts, which is associated with N-methyl-d-aspartate (NMDA) glutamate receptor-mediated elevations in intracellular Ca2+. Both spontaneous and induced activation of dorsal root ganglion (DRG) neurons, manifested by action potentials and TTX-dependent inward currents, significantly reduced fibroblast GJC in Neuron/Fibroblast (N/F) cocultures. Reduced fibroblast GJC by DRG neurons was prevented by blockade of NMDA receptors and decrease of intra- and intercellular Ca2+. Immunocytochemistry showed that the NR1 subunit of the NMDA receptor coexisted with CX43 at the plasma membrane of fibroblasts. Moreover, glutamate applied to fibroblast cultures triggered NMDA receptor-dependent intracellular Ca2+ elevations and decrease of GJC. These data demonstrate that NMDA receptor activation contributes to downregulation of GJC in fibroblasts. Since fibroblasts have been shown to facilitate DRG neurite growth and survival, our findings provide a glimpse at the impact that neuronal activation has on fibroblast networks.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1280, 14 July 2009, Pages 13–22
نویسندگان
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