کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4328553 | 1614180 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hydrogen gas is ineffective in moderate and severe neonatal hypoxia-ischemia rat models
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کلمات کلیدی
PBSTTCMCAOMDA2,3,5-triphenyltetrazolium chloride - 2،3،5-trihenyltetrazolium chloridemiddle cerebral artery occlusion - انسداد شریان (سرخرگ) مغزی میانیNeonatal hypoxia–ischemia - ایسکمی هیپوکسی نوزادانFree radical - رادیکالهای آزاد Phosphate buffered saline - فسفات بافر شورmalondialdehyde - مالون دی آلدهیدhypoxia–ischemia - هیپوکسی-ایسکمیHydrogen gas - گاز هیدروژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Hydrogen gas (H2) has been shown to ameliorate brain injury in experimental adult rat focal ischemia and in a mild neonatal hypoxia-ischemia (HI, 90 min hypoxia) rat model. In this study we tested H2 in moderate (120 min hypoxia) and severe (150 min hypoxia) neonatal HI rat models. We hypothesized that H2 would improve outcomes after neonatal HI by scavenging free radicals. Two hundred (200) unsexed Sprague-Dawley rats at day 10 of life (p10) underwent neonatal HI with the Rice-Vannucci model. Multiple treatment protocols were studied, including pre-ischemic treatment, intra-ischemic treatment, and post-ischemic treatment (Sham n = 32, HI n = 82, HI + H2n = 86). We also tested H2 in middle cerebral artery occlusion (MCAO) in adult rats (MCAO n = 9, MCAO + H2n = 7) for comparison. Analysis at 24 h included infarction volume, measurement of brain concentration of malondialdehyde (MDA) (an end-product of lipid peroxidation), daily weight, Nissl histology, and mortality. In moderate and severe neonatal HI models, hydrogen gas therapy (2.9% concentration H2) was not associated with decreased volume of infarction or decreased concentration of MDA. H2 gas pretreatment (2.9%) was associated with increased infarction volume in neonatal HI. In MCAO in adult rats, H2 gas therapy demonstrated a trend of beneficial effect. Exposure of H2 gas to non-ischemic neonates resulted in a significant increase in brain concentration of MDA. We conclude that 2.9% H2 gas therapy does not ameliorate moderate to severe ischemic damage in neonatal hypoxia-ischemia.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1259, 9 March 2009, Pages 90-97
Journal: Brain Research - Volume 1259, 9 March 2009, Pages 90-97
نویسندگان
Gerald A. Matchett, Nancy Fathali, Yu Hasegawa, Vikram Jadhav, Robert P. Ostrowski, Robert D. Martin, Ihab R. Dorotta, Xuejun Sun, John H. Zhang,