کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4329090 | 1614205 | 2008 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inactivation of median preoptic nucleus causes c-Fos expression in hypocretin- and serotonin-containing neurons in anesthetized rat
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کلمات کلیدی
5-HTPOAVLPOTBSGADaCSFHcrtDRNMCHgamma-aminobutyric acid - اسید گاما آمینو بوتیریکOrexin - اورکسینTris buffer saline - بیس بال TrisSleep - خوابSerotonin - سروتونینserotonin (5-hydroxytryptamine) - سروتونین (5-hydroxytryptamine)Fos-ir - فوزیartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیPreoptic area - منطقه Preopticventrolateral preoptic area - منطقه پرپتیک ventrolateraldorsal raphe nucleus - هسته رافهmedian preoptic nucleus - هسته پیشوپتیک مدیاستmelanin-concentrating hormone - هورمون متمرکز کننده ملانینHypocretin - هیپوکراتینGABA - گابا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Inactivation of median preoptic nucleus causes c-Fos expression in hypocretin- and serotonin-containing neurons in anesthetized rat Inactivation of median preoptic nucleus causes c-Fos expression in hypocretin- and serotonin-containing neurons in anesthetized rat](/preview/png/4329090.png)
چکیده انگلیسی
The median preoptic nucleus (MnPN) of the hypothalamus contains sleep-active neurons including sleep-active GABAergic neurons and is involved in the regulation of nonREM/REM sleep. The hypocretinergic (HCRT) neurons of the perifornical-lateral hypothalamic area (PF-LHA) and serotonergic (5-HT) neurons of the dorsal raphe nucleus (DRN) are mostly active during waking and have been implicated in the regulation of arousal. MnPN GABAergic neurons project to the PF-LHA and DRN. It is hypothesized that MnPN promotes sleep by inhibiting multiple arousal systems including HCRT and other wake-active neurons within the PF-LHA and 5-HT neurons in the DRN. We examined the effects of inactivation of MnPN neurons by locally microinjecting 0.2 µl of 1 mM or 10 mM solutions of a GABAA receptor agonist, muscimol, into the MnPN on Fos expression (Fos-IR) in the PF-LHA neurons including HCRT neurons and 5-HT neurons in the DRN in anesthetized rats. Compared to artificial cerebrospinal fluid control, microinjection of muscimol into the MnPN resulted in significantly higher percentages of HCRT and non-HCRT neurons in the PF-LHA and 5-HT neurons in the DRN that exhibited Fos-IR. The percentage of melanin-concentrating hormone (MCH)+/Fos+ neurons in the PF-LHA did not change after muscimol treatments. These results support a hypothesis that the activation of MnPN neurons contributes to the suppression of wake-promoting systems including HCRT and other unidentified neurons in the PF-LHA and 5-HT neurons in the DRN. These results also suggest that MCH neurons may not be under MnPN inhibitory control. These findings are consistent with a hypothesized role of MnPN in sleep regulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1234, 9 October 2008, Pages 66-77
Journal: Brain Research - Volume 1234, 9 October 2008, Pages 66-77
نویسندگان
Sunil Kumar, Ronald Szymusiak, Tariq Bashir, Natalia Suntsova, Seema Rai, Dennis McGinty, Md. Noor Alam,