کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4330221 | 1614254 | 2007 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gene profiling of pooled single neuronal cell bodies from laser capture microdissected vervet monkey lateral geniculate nucleus hybridized to the Rhesus Macaque Genome Array
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کلمات کلیدی
FDRLCMqRT-PCRLGNGene expression - بیان ژنlaser capture microdissection - لیزر ضبط میکرو دیسکسیونfalse discovery rate - میزان کشف کاذبMonkey - میمونlateral geniculate nucleus - هسته ژنیکول جانبی جانبیquantitative real-time reverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز معکوس در زمان واقعی معکوس
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
This report is based on an ongoing study to examine gene expression differences in monkey lateral geniculate nucleus (LGN). Here, samples from an Old World species, the vervet monkey (Cercopithecus aethiops), were cross-hybridized to the Rhesus Macaque Genome Array (Affymetrix). Microarray analysis was performed using laser capture microdissected populations of individual neuronal cell bodies isolated from the LGN compared to heterogeneous samples from whole lamina. Our results indicated that cross-species hybridization of microdissected brain tissue samples from vervet monkeys to the Rhesus array produced reliable and biologically relevant data sets. We present the first list of genes enriched in the large neuronal cell bodies of the LGN. We found that these cell bodies are concentrated with genes involved in metabolic processes and protein synthesis, whereas signaling molecules including chemokines and integrins were expressed at higher levels within heterogeneous samples. Our data set also provides support for a contribution of Wnt signaling in adult monkey LGN.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1185, 14 December 2007, Pages 33-44
Journal: Brain Research - Volume 1185, 14 December 2007, Pages 33-44
نویسندگان
Pascal E.D. Lachance, Avi Chaudhuri,