کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4331679 1614309 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of (--)-epigallocatechin-3-gallate on the activity of substantia nigra dopaminergic neurons
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Effects of (--)-epigallocatechin-3-gallate on the activity of substantia nigra dopaminergic neurons
چکیده انگلیسی

Despite many studies on the biological and pharmacological properties of (−)-epigallocatechin-3-gallate (EGCG), an active component of green tea, information on neuronal modulation by EGCG is limited. This study was designed to investigate the effects of EGCG on the electrical activity of rat substantia nigra dopaminergic neurons using whole-cell patch clamp recordings. The spike frequency was increased to 6.33 ± 0.23 (p < 0.05) and 7.15 ± 0.29 (p < 0.05) by 5 and 10 μM EGCG, respectively, from the control level of 5.49 ± 0.19 spikes/second, respectively (n = 18). The resting membrane potential of the cells was decreased to − 45.66 ± 0.45 and − 43.99 ± 0.87 (p < 0.05), by 5 and 10 μM EGCG, respectively, from − 47.82 ± 0.57 mV. The amplitude of afterhyperpolarization was decreased to 12.73 ± 0.45 (p < 0.05) and 11.60 ± 0.57 (p < 0.05) by 5 and 10 μM EGCG, respectively, from 13.80 ± 0.31 mV. The neuronal activity of dopaminergic neurons is closely linked to dopamine release. When neurons switch from a single-spike firing to bursts of action potentials, the release of dopamine increases. The above experimental results suggest that EGCG increases the neuronal activity via inhibition of calcium-dependent potassium currents underlying the afterhyperpolarization, and it could act as a facilitating factor that elicits NMDA-dependent bursts of action potentials like apamin or bicuculline methiodide.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1130, 26 January 2007, Pages 114–118
نویسندگان
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