کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4333103 1292921 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Differential expression of the estrogen receptors alpha and beta during postnatal development of the rat cerebellum
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Differential expression of the estrogen receptors alpha and beta during postnatal development of the rat cerebellum
چکیده انگلیسی

Estrogen receptor (ER) β is a dominant ER subtype in the adult cerebellum. However, it is not known if this is also the case for the developing cerebellum. In the present study, quantitative real-time RT-PCR demonstrated that levels of cerebellar ERα mRNA in neonatal pups were significantly higher than in adults. In contrast, expression levels of cerebellar ERβ mRNA remained significantly unchanged during postnatal development. In situ hybridization and immunohistochemistry demonstrated that ERα mRNA and protein were predominantly expressed by Purkinje cells at all ages examined. ERα-expressing Purkinje cells were confined to the anterior lobes at postnatal day 7 (P7) but distributed in most lobes at P14 and P21. In the adult cerebellum, however, only a few ERα-immunoreactive Purkinje cells were observed. Thus, ERα expression was transiently increased during the time when Purkinje cell dendritic growth and synapse formation proceed, suggesting that a role for ERα in Purkinje cell differentiation. ERβ expression occurred in Golgi type neurons in the granular layer at P7, Purkinje cells at P14, and basket cells in the molecular layer at P21 and was detected in all the cell types in the adult cerebellum, suggesting a role for ERβ associated with neuronal differentiation and maintenance. Furthermore, double-labeled immunofluorescence for ERα and ERβ demonstrated their colocalization in Purkinje cells at P14, suggesting a possibility of their interaction. The discrete expression profiles for ERα and ERβ in the developing cerebellum suggest the two ERs play distinct roles in cerebellar development.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1083, Issue 1, 14 April 2006, Pages 39–49
نویسندگان
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