کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4335242 | 1295140 | 2011 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Microbore liquid chromatography with UV detection to study the in vivo passage of compound 21, a non-peptidergic AT2 receptor agonist, to the striatum in rats Microbore liquid chromatography with UV detection to study the in vivo passage of compound 21, a non-peptidergic AT2 receptor agonist, to the striatum in rats](/preview/png/4335242.png)
A microbore liquid chromatography method coupled to UV detection was developed and validated in order to monitor the passage of compound 21 (C21), a non-peptide angiotensin II type 2 receptor agonist, to the striatum of rats. For this purpose, sampling from the striatum was performed using the in vivo microdialysis technique. Separations were performed on a C18 microbore (1 mm i.d.) column using gradient elution. The retention time for C21 was found to be 6.3 min. The calibration curve was linear between 10 and 200 ng/ml with a correlation coefficient ≥0.999. The limit of detection and the limit of quantification were 3 and 10 ng/ml respectively. The intra-day and the inter-day precision (RSD%) ranged between 0.5 and 4.6% with an average recovery of 101.5 ± 10.0% (mean ± SD, n = 15). In vivo experiments were performed on rats to measure the concentration of C21 in striatal dialysates after intraperitoneal (10 or 50 mg/kg) or intravenous injection (10 mg/kg or 20 mg/kg) of C21 and suggest minimal passage of the compound to the striatum.
► Compound 21 is a non-peptide angiotensin II type 2 receptor agonist.
► We developed and validated a microbore liquid chromatography method to monitor compound 21.
► Sampling was performed by in vivo microdialysis.
► We studied the passage of compound 21 to the striatum after system administration.
► We observed minimal passage to the striatum.
Journal: Journal of Neuroscience Methods - Volume 202, Issue 2, 15 November 2011, Pages 137–142