Western blot detection of brain phosphoproteins after performing Laser Microdissection and Pressure Catapulting (LMPC)

► The anatomical complexity of the CNS allows highly specific brain-cell responses. ► Specific brain proteomic responses occur within functional brain sub-regions. ► The fine-tuning of kinase activities is critical for brain neural functions. ► The characterization of kinase-mediated neural changes in brain areas is complex. ► LMPC/Western blot is a method of choice to obtain accurate CNS kinase profiles.