کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4335892 1295188 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Systemic RNA interference for the study of learning and memory in an insect
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Systemic RNA interference for the study of learning and memory in an insect
چکیده انگلیسی

RNA interference (RNAi) is a powerful technique for the study of molecular mechanisms underlying many biological processes, including brain functions. Among methods for RNAi, systemic administration of double-stranded RNA (systemic RNAi) is the most convenient for basic research as well as medical application, but it has yielded only limited success. To our knowledge, systemic RNAi has not been achieved for the study of learning and memory in any animals. Here we demonstrate successful systemic RNAi of the NOS gene coding for nitric oxide synthase, which, as we previously suggested, plays a critical role in the formation of olfactory long-term memory (LTM), in the nymphal cricket Gryllus bimaculatus. In situ hybridization demonstrated a high level of expression of NOS in a subset of Kenyon cells of the mushroom body, which is known to participate in olfactory learning and memory, in addition to some neurons around the antenna lobe and the base of the optic lobe. Injection of NOS double-stranded RNA (dsRNA) into the haemolymph completely impaired 1-day memory retention, although 30 min retention was unaffected. This impairment was fully rescued by injection of an NO donor, NOR3, thus suggesting that the effect of NOS dsRNA is through inhibition of NOS. Inhibition of NOS had no effects on recall of LTM. The results demonstrate that silencing of NOS expression by systemic RNAi impairs LTM formation. Systemic RNAi will become a useful method for study of the molecular mechanisms of learning and memory.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 179, Issue 1, 30 April 2009, Pages 9–15
نویسندگان
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