کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4336446 1295213 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Primary cultures of nervous system cells from the larva of the ascidian Ciona intestinalis
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Primary cultures of nervous system cells from the larva of the ascidian Ciona intestinalis
چکیده انگلیسی

The ascidian Ciona intestinalis is a useful model for the study of nervous system development and function. The larva of this animal represents a ‘primitive’ vertebrate form that contains only about 100 neurons in the CNS. Although embryos can be easily subjected to genetic manipulation, the nervous system cells are not easily accessible for neurophysiological study at the larval stage. To remedy this problem, we have developed a method to obtain primary cell cultures from the larval stage of Ciona. Light microscopy and electrophysiology discriminate several types of cells including neurons and photoreceptors. The results show that in Ciona primary cultures different types of neurons as well as neurite sprouting and synapse formation can be visualised. Ciona primary cell cultures will be very useful to study the biochemical, molecular and biophysical properties of individual cells in the larval nervous system of C. intestinalis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 165, Issue 2, 30 September 2007, Pages 191–197
نویسندگان
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