کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4338290 1614854 2012 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Delayed increase of astrocytic aquaporin 4 after juvenile traumatic brain injury: Possible role in edema resolution?
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Delayed increase of astrocytic aquaporin 4 after juvenile traumatic brain injury: Possible role in edema resolution?
چکیده انگلیسی

Traumatic brain injury (TBI) is one of the leading causes of death and disability in children and adolescents. The neuropathological sequelae that result from TBI are a complex cascade of events including edema formation, which occurs more frequently in the pediatric than the adult population. This developmental difference in the response to injury may be related to higher water content in the young brain and also to molecular mechanisms regulating water homeostasis. Aquaporins (AQPs) provide a unique opportunity to examine the mechanisms underlying water mobility, which remain poorly understood in the juvenile post-traumatic edema process. We examined the spatiotemporal expression pattern of principal brain AQPs (AQP1, AQP4, and AQP9) after juvenile TBI (jTBI) related to edema formation and resolution observed using magnetic resonance imaging (MRI). Using a controlled cortical impact in post-natal 17 day-old rats as a model of jTBI, neuroimaging analysis showed a global decrease in water mobility (apparent diffusion coefficient, ADC) and an increase in edema (T2-values) at 1 day post-injury, which normalized by 3 days. Immunohistochemical analysis of AQP4 in perivascular astrocyte endfeet was increased in the lesion at 3 and 7 days post-injury as edema resolved. In contrast, AQP1 levels distant from the injury site were increased at 7, 30, and 60 days within septal neurons but did not correlate with changes in edema formation. Group differences were not observed for AQP9. Overall, our observations confirm that astrocyticAQP4 plays a more central role than AQP1 or AQP9 during the edema process in the young brain.


► Diffuse edema formation in a rodent model of juvenile traumatic brain injury.
► Astrocytic AQP4 expression parallels the edema resolution after jTBI.
► Neuronal AQP1 expression is increased after jTBI.
► Long-term changes of AQP4 and AQP1 after jTBI.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 222, 11 October 2012, Pages 366–378
نویسندگان
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