کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4338488 1614870 2012 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of amphetamine place conditioning on excitatory synaptic events in the basolateral amygdala ex vivo
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Effect of amphetamine place conditioning on excitatory synaptic events in the basolateral amygdala ex vivo
چکیده انگلیسی

The basolateral amygdala (BLA) plays an important role in the formation of associations between context and drug. BLA activity and BLA-dependent drug-seeking behavior are driven by excitatory inputs. Drug-seeking behavior driven by context involves participation of the BLA, and plasticity of excitatory inputs to the BLA may contribute to this behavior. In this study, amphetamine conditioned place preference (AMPH CPP) was used to model the formation of context-drug associations. Learning-induced changes of excitatory synapses within the BLA were examined. Male Sprague–Dawley rats were assigned to one of three groups, the experimental group (AMPH CPP) or one of two control groups (saline or AMPH delayed pairing). Approximately 24 h after testing their preference, spontaneous and miniature excitatory postsynaptic currents (sEPSCs and mEPSCs, respectively) in BLA pyramidal neurons were investigated using whole-cell patch-clamp recordings. There were no between-groups differences in the amplitude or frequency of sEPSCs or mEPSCs. In a higher osmolarity solution to increase release, there was a significantly greater frequency of the mEPSCs in neurons from AMPH CPP animals compared with controls. This was observed with no change detected in the probability of glutamate release. Together, these data demonstrate no evidence for increased synaptic strength, but are consistent with an increase in the number of synapses in the BLA after AMPH CPP. These findings may underlie increased excitatory drive of the BLA after AMPH CPP, and contribute to the animals' preference for the AMPH-paired compartment.

▶No changes in the spontaneous or miniature EPSCs in BLA after AMPH CPP. ▶No presynaptic changes in the probability of glutamate release after AMPH CPP. ▶Significant increase in frequency of mEPSCs in high sucrose solution after AMPH CPP. ▶Drug-seeking behavior associated with increased BLA synapse number, not strength.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 206, 29 March 2012, Pages 7–16
نویسندگان
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