کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4338700 1614877 2011 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Differential expression, alternative splicing, and adhesive properties of the zebrafish δ1-protocadherins
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Differential expression, alternative splicing, and adhesive properties of the zebrafish δ1-protocadherins
چکیده انگلیسی

Protocadherins comprise the largest family within the cadherin superfamily of cell surface receptors. Here, we characterize the δ1-protocadherin subfamily during the development of the zebrafish nervous system. In zebrafish, there are five δ1-protocadherins: pcdh1a, pcdh1b, pcdh7a, pcdh7b, andpcdh9. Each protocadherin gene is highly homologous to its human ortholog. While the expression pattern in the developing CNS is similar for each δ1-protocadherin, with labeling observed in all major subdivisions, the detailed patterns are distinct. In addition, we provide evidence for alternative splicing of the pcdh7b and pcdh9 genes, resulting in variation in their respective cytoplasmic domains. As protocadherins are widely regarded to act as cell adhesion molecules, we used in vitro assays of δ1-pcdh ectodomains to directly test their adhesive properties. We found no evidence for calcium-dependent, homophilic adhesion, contrasting sharply with the behavior of classical cadherins.

▶Zebrafish have five δ1-protocadherins. ▶pcdh1 and pcdh7 are duplicated in zebrafish. ▶Zebrafish do not have a pcdh11 ortholog. ▶δ1-Protocadherins are mosaically expressed in the retina. ▶Zebrafish δ1-pcdhs do not exhibit calcium-dependent adhesion.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 199, 29 December 2011, Pages 523–534
نویسندگان
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