کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4340231 | 1295788 | 2009 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The retinoic acid inducible Cas-family signaling protein Nedd9 regulates neural crest cell migration by modulating adhesion and actin dynamics
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کلمات کلیدی
ECMCas-LDRGeGFPHEF1PDLISHNCCdorsal root ganglion - گانگلیون ریشه پشتیIn situ hybridization - Hybridization در محلSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAoptic density - تراکم نورRetinoic acid - رتینوئیک اسیدneural crest cell - سلول های خونی عصبیExtracellular matrix - ماتریکس خارج سلولیenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته استPoly-d-lysine - پلی دی لیزینCytoskeleton - چارچوب یاخته، سیتواسکلتون، اسکلت سلولیFocal adhesion - چسبندگی کانونیMultipotency - چند ضلعی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The retinoic acid inducible Cas-family signaling protein Nedd9 regulates neural crest cell migration by modulating adhesion and actin dynamics The retinoic acid inducible Cas-family signaling protein Nedd9 regulates neural crest cell migration by modulating adhesion and actin dynamics](/preview/png/4340231.png)
چکیده انگلیسی
Cell migration is essential for the development of numerous structures derived from embryonic neural crest cells (NCCs), however the underlying molecular mechanisms are incompletely understood. NCCs migrate long distances in the embryo and contribute to many different cell types, including peripheral neurons, glia and pigment cells. In the present work we report expression of Nedd9, a scaffolding protein within the integrin signaling pathway, in non-lineage-restricted neural crest progenitor cells. In particular, Nedd9 was found to be expressed in the dorsal neural tube at the time of neural crest delamination and in early migrating NCCs. To analyze the role of Nedd9 in neural crest development we performed loss- and gain-of-function experiments and examined the subsequent effects on delamination and migration in vitro and in vivo. Our results demonstrate that loss of Nedd9 activity in chick NCCs perturbs cell spreading and the density of focal complexes and actin filaments, properties known to depend on integrins. Moreover, a siRNA dose-dependent decrease in Nedd9 activity results in a graded reduction of NCC's migratory distance while forced overexpression increases it. Retinoic acid (RA) was found to regulate Nedd9 expression in NCCs. Our results demonstrate in vivo that Nedd9 promotes the migration of NCCs in a graded manner and suggest a role for RA in the control of Nedd9 expression levels.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 162, Issue 4, 15 September 2009, Pages 1106-1119
Journal: Neuroscience - Volume 162, Issue 4, 15 September 2009, Pages 1106-1119
نویسندگان
J.B. Aquino, F. Lallemend, F. Marmigère, I.I. Adameyko, E.A. Golemis, P. Ernfors,