کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4341514 | 1295838 | 2006 | 13 صفحه PDF | دانلود رایگان |
Potassium channels contribute to basic neuronal excitability and modulation. Here, we examined expression patterns of the voltage-gated potassium channel Kv1.4, the nociceptive transduction channels TRPV1 and TRPV2 as well as the putative anti-nociceptive cannabinoid receptor CB1 by immunofluorescence double-labelings in sections of rat dorsal root ganglia (DRGs).Kv1.4, TRPV1 and CB1 were each detected in about one third of neurons (35.7±0.5%, 29.4±1.1% and 36.4±0.5%, respectively, mean diameter 19.1±0.3 μm). TRPV2 was present in 4.4±0.4% of all neurons that were significantly larger in diameter (27.4±0.7 μm; P<0.001). Antibody double-labeling revealed that the majority of Kv1.4-positive neurons co-expressed TRPV1 (73.9±1.5%) whereas none expressed TRPV2. The largest overlap was found with CB1 (93.1±0.1%). CB1 expression resembled that seen for Kv1.4 since the majority of neurons expressing CB1-protein also expressed TRPV1 (69.4±6.5%) but not TRPV2 (0.6±0.3%). When CB1-mRNA was detected using in situ hybridizations an additional subset of larger neurons was labeled including 82.4±17.7% of the TRPV2 expressing neurons. However, co-localization of Kv1.4 with CB1-mRNA (92%, mean diameter: 18.5 μm) was essentially the same as with CB1-protein.The almost complete overlap of CB1 and Kv1.4 in nociceptive DRG neurons suggests a functional synergistic action between Kv1.4 and CB1. The potassium channel may have two important roles in nociception. As the molecular basis of A-type current it could be involved in the control of repetitive discharges at peripheral terminals and as a downstream signal transduction site of CB1 in the control of presynaptic transmitter release at central terminals.
Journal: Neuroscience - Volume 142, Issue 2, 13 October 2006, Pages 527–539