کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4341982 | 1295852 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Physiology and morphology of callosal projection neurons in mouse
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کلمات کلیدی
PBSIR-DICHRPPSPaCSFBDAinterhemispheric - interemisphericISI - آی اس آیbiotinylated dextran amine - بیوترنیته دیستران آمینanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of variancenumerical aperture - دیافراگم عددیinter-spike interval - فاصله بین سنبلهBarrel cortex - قشر بشکهartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریCortical circuits - مدارهای قورباغهpostsynaptic potential - پتانسیل پتانسیپتیکHorseradish peroxidase - پراکسیداز هوررادیشinfrared differential interference contrast - کنتراست تداخل اختلاط مادون قرمز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
In the mammalian neocortex, the corpus callosum serves as the major source of interhemispheric communication, composed of axons from callosal neurons located in supragranular (II/III) and infragranular (V/VI) layers. We sought to characterize the physiology and morphology of supragranular and infragranular callosal neurons in mice using retrograde tracers and whole-cell patch clamp recordings. Whole-cell patch clamp recordings were made from retrogradely labeled callosal neurons following unilateral injection of fluorescent latex microspheres in the contralateral sensory-motor cortex. Following recordings and biocytin dialysis, labeled neurons were reconstructed using computer-assisted camera lucida (Neurolucida) for morphological analyses. Whole-cell recordings revealed that callosal neurons in both supra- and infragranular layers display very similar intrinsic membrane properties and are characteristic regular-spiking neurons. Morphological features examined from biocytin-filled reconstructions as well as retrogradely BDA labeled cells did not reveal any differences. Analysis of spontaneous postsynaptic potentials from callosal neurons did reveal several differences including average amplitude, frequency, and decay time. These findings suggest that callosal neurons in both supra- and infragranular layers have similar phenotypes though belong to different local, intracortical networks.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 153, Issue 3, 15 May 2008, Pages 654-663
Journal: Neuroscience - Volume 153, Issue 3, 15 May 2008, Pages 654-663
نویسندگان
R.L. Ramos, D.M. Tam, J.C. Brumberg,