کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4343272 1615088 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Intraventricular infusion of a low fraction of serum enhances neurogenesis and improves recovery in a rodent stroke model
ترجمه فارسی عنوان
تزریق داخل وریدی یک کسر کم سرم موجب افزایش نوروژنز و بهبودی در یک مدل سکته مغزی می شود
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


• A serum fraction (100K) was easily derived from serum.
• 100K/bFGF enhanced cell proliferation at SVZ area and infarcted brain.
• 100K/bFGF increased the number of MAP-2 cells at infarcted brain in MCAO rat.
• 100K/bFGF improved animals’ motor coordination of MCAO rat.

Enhancing endogenous neurogenesis is a potential therapeutic strategy in stroke treatment. We have previously demonstrated that treatment with a fraction of serum with molecular weight of less than 100 kDa (100K) combined with bFGF promoted neurogenesis of cultured stem and progenitor cells (NSPCs). In this study, we further evaluated the efficacy of intraventricular administration of 100K with bFGF (100K/bFGF) in a rat model of transient middle cerebral artery occlusion (MCAO). Rats administered 100K/bFGF on post-stroke day 1 exhibited a higher number of Ki67 and Nestin immunoreactive cells at the subventricular zone (SVZ) area and in the infarcted brain, indicating promotion of NSPCs proliferation. The 100K/bFGF treatment also predominantly increased the number of MAP-2 immunoreactive cells rather than GFAP immunoreactive cells at the SVZ area and in the infarcted regions, implying that 100K/bFGF dominated NSPCs differentiating into neurons rather than astrocytes. Importantly, treatment with 100K/bFGF significantly improved the animals’ motor coordination. These findings demonstrated that treatment with a low serum fraction and bFGF benefited ischemic stroke likely through promotion of the proliferation and neuronal differentiation of endogenous NSPCs.

The cellular distribution of ischemia rat with or without the treatment of 100 K/bFGF.Figure optionsDownload high-quality image (320 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 611, 12 January 2016, Pages 14–20
نویسندگان
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